| |
|
|
| Registros recuperados : 15 | |
| 1. |  | Diaz, A. Producción de inoculantes en Uruguay ln: Reunión Latinoamericana sobre Inoculantes para Leguminosas, 1 : 1964 ago 10-13 : Montevideo [Memorias]. Montevideo (Uruguay): s.l., 1964. s.p.| Biblioteca(s): INIA La Estanzuela. |
|   |
| 3. | | DÍAZ, A.; LANFRANCO, B. Qué factores afectan el consumo de manzanas y peras en Uruguay?. Revista INIA Uruguay, 2019, no. 57, p. 61-66. (Revista INIA; 57) El articulo está basado en la tesis de la Maestría en Economía de la FCCEE (UdelaR) titulada ?Consumo de frutas: estimación y análisis de sus determinantes para lograr una ingesta acorde a la recomendación internacional ?, del Ec. Andrés...| Biblioteca(s): INIA Las Brujas. |
|  |
| 8. | | MIRABALLES, C.; BUSCIO, D.; SARAVIA, A.; DIAZ, A.; CASTRO-JANER, E. Comportamiento de vacas en ordeñe ante una alternativa de control no químico de la "Mosca de los cuernos" , Haematobia Irritans. In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. p.219-221.| Biblioteca(s): INIA La Estanzuela. |
| |
| 11. | | CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación...| Biblioteca(s): INIA Las Brujas. |
|  |
| 14. | | Izaguirre de Artucio, P.; Díaz, A.; Millot, J.C.; Mazzella, C.; Rivas, M.; Grun, S.; Davies, P.; Baycé, D.; Pereira, J. Proyecto : estudio de los recursos geneticos de Bromus y paspalum ln: Seminario Nacional de Campo Natural, 2 : 1990 nov 15-16 : Tacuarembó Montevideo (Uruguay): Hemisferio Sur, 1990. p157-158 Participaron en el seminario : INIA, Sociedad Uruguaya de Pasturas Naturales, Facultad de Agronomía, Plan Agropecuario| Biblioteca(s): INIA La Estanzuela; INIA Tacuarembó. |
| |
| 15. | | ALGORTA, I.; AZAMBUJA, T.; DÍAZ, A.; ERRO, L.; ESTABLE, L.; GONZÁLEZ, G.; HERWIG, G.; MARTÍNEZ, J.; MORIYAMA, S.; OLAIZOLA, V.; OSORIO, F.; PACHECO, P.; PÉREZ, C.; SANTÍN, V.; TRUJILLO, M.; VODANOVICH, V.; ZELMONOVICH, C. Grupo interinstitucional de promoción del consumo saludable de frutas y verduras. (Abs.1, Sección: Generales). IN: CONGRESO NACIONAL DE HORTI-FRUTICULTURA, 13. AÑO DE LA AGRICULTURA FAMILIAR DE LA FAO. "SOSTENIBILIDAD DE LA PRODUCCIÓN HORTIFRUTÍCOLA FAMILAR". 3-6 SETIEMBRE 2014, MONTEVIDEO (UY). Trabajos presentados. Montevideo (UY): INIA; SUHF, 2014. p. 17| Biblioteca(s): INIA Las Brujas. |
| |
| Registros recuperados : 15 | |
|
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha actual : |
30/06/2021 |
|
Actualizado : |
30/06/2021 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
|
Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
|
Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
|
ISSN : |
1877-959X |
|
DOI : |
10.1016/j.ttbdis.2021.101747 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
|
Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
|
Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
|
Asunto categoría : |
L73 Enfermedades de los animales |
|
Marc : |
LEADER 03773naa a2200265 a 4500
001 1062207
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
022 $a1877-959X
024 7 $a10.1016/j.ttbdis.2021.101747$2DOI
100 1 $aCASTRO JANER, E.
245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com
520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021
653 $aAcaricides
653 $aIxodidae
653 $aReaxys retrieves substances
653 $aRhipicephalus microplus
700 1 $aDÍAZ, A.
700 1 $aFONTES, F.
700 1 $aBARAIBAR, F.
700 1 $aSAPORITI, T.
700 1 $aOLHAGARAY, M. E.
773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
